The determination of acetaldehyde in blological samples by head-space gas chromatography

✍ Scribed by C.J.Peter Eriksson; Helmuth W. Sippel; Olof A. Forsander

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 501 KB
• Volume: 80
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90631-5

✦ Synopsis

A method for the determination of acetaldehyde (AcH) in biological samples by head-space gas chromatography is presented. Human venous blood (antecubital), rat blood (heart-punctured) rat liver (freeze-clamped), and rat and mouse brain (freeze-clamped) were used as the biological samples. The method involves two steps, in the first of which the samples are deproteinized with perchloric acid (PCA). Rat blood can, alternatively, be hemolyzed with water. In the second step, the deproteinized supematant or hemolyzed blood is pipetted into a serum bottle, which is sealed with a rubber stopper and brought to 65°C in a sampling turntable. Head-space samples are then automatically taken for GLC analysis. Ethanol causes a nonenzymatic formation of AcH in the PCA supematants of liver and brain, which can be inhibited by the use of thiourea. This reaction is minor in the blood supematants and cannot be inhibited there by thiourea. The method described measures the total AcH content without regard to any binding. Some of the AcH in rat blood was shown to be bound.