𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The determination of 2′-O-methylnucleosides in RNA

✍ Scribed by Lee M. Pike; Fritz Rottman

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
714 KB
Volume
61
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid and sensitive procedure is described for determining the 2'-0methylnucleoside composition of an RNA sample. The RNA is enzymatically hydrolyzed to nucleosides and the 2'-0-methylnucleoside fraction is isolated by DEAE-cellulose (borate) column chromatography. Boric acid is removed as its methyl ester and the 2'-0-methylnucleosides are resolved by liquid chromatography in the presence of ethylene glycol. The sensitivity of this method is sufficient to distinguish RNA samples which differ only 2-37~ in 2'-O-methylnucleoside composition.

📜 SIMILAR VOLUMES

Determination of 2′-O-methyl groups in 3
Determination of 2′-O-methyl groups in 32P-labeled RNAs
✍ I. Klein; J. Klootwijk 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 742 KB

A two-dimensional method for the detection of ribose methyl groups in 32P-labeled RNAs is described which is suitable for both qualitative and quantitative purposes. The method involves fractionation of an alkaline hydrolysate of the RNA by two-dimensional electrophoresis of the digest on DEAEcellul