✦ LIBER ✦

The detection of DNA polymerase activity in the diagnosis of HBV infection

✍ Scribed by Dr. Colin R. Howard

Publisher: John Wiley and Sons
Year: 1978
Tongue: English
Weight: 328 KB
Volume: 3
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890030116

No coin nor oath required. For personal study only.

✦ Synopsis

During recent years considerable attention has been directed to the analysis of the 42-nm double-shelled particle present in some sera from individuals infected with hepatitis B virus. Kemoval of the outer surface antigen with nonionic detergent reveals an inner core antigen-reactive component 27 nm in diameter which closely resembles a small icosdiedral virus particle in morphology [Almeida et al, 1971). In addition, exposure of the core component activates a unique nucleic acid polymerase activity in thosc particles containing a circular DNA molecule which provides the enzyme with an endogenous template [Kaplan et al, 19731. The nature of the presumptive hepatitis B virus genome pre-

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