The destructive effect of 2,6-diaminopurine of kappa of stock 51 killers, variety 4, of paramecium aurelia
✍ Scribed by Jacobson, Winifred E. ;Williamson, Mary ;Stock, C. Chester
- Publisher
- John Wiley and Sons
- Year
- 1952
- Tongue
- English
- Weight
- 759 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Certain stocks of Paramecium aurelia, known as "killers, " differ from others in that they produce a poison, paramecin (Sonneborn, Dippell and Jacobson, '46, '47), which is toxic to other non-paramecin producing stocks known as " sensitives. " A killer must always contain the cytoplasmic factor, kappa, and the killer (K) gene ; kappa is gene dependent, but not gene initiated (Sonneborn, '43, '47, '50a), It is particulate ; its size is 0.2 to 0.8 p ; it is composed, at least in part, of desoxyribonucleic acid ; it reproduces at rates which may differ from those of the paramecium containing it; and hundreds to over a thousand of these entities may be found in the cytoplasm of a single killer animal (Preer, '48, '50). Paramecin contains desoxyribonucleoprotein as an important constituent (Van Wagtendonk, '48). It also is particulate, one particle killing one sensitive animal (Sonneborn, Jacobson and Dippell, '46; Austin, '46, '48).
Recently, several methods for reducing kappa concentration have been reported. Temperatures between 30" and 38°C. will reduce the number of kappa particles per animal or cause the complete loss of kappa (Sonneborn, '46 ; Sonneborn, Dippell and Jacobson, '47). X-rays (Preer, '48b, '50), nitrogen mustard (Gecklei-, '49), certain antibiotics (Brown, '50 ; 'We wish to acknowledge support of this study by funds from the American Cancer Society.