The derivatization of oxidized polysaccharides for protein immobilization and affinity chromotography
β Scribed by JUNOWICZ, E; CHARM, S
- Book ID
- 122977375
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 538 KB
- Volume
- 428
- Category
- Article
- ISSN
- 0304-4165
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## Abstract An oxidized porous Si interferometer was used to measure binding of immunoglobulin G (IgG) to an immobilized protein A capture probe. Protein A was nonβcovalently immobilized on a thermally oxidized porous Si (PSiO~2~) sample and exposed to IgG originating from different species. The re
Affinity systems based on specific molecular recognition are valuable tools for detection, purification and immobilization of recombinant proteins. Here, novel multipartite affinity fusion vectors were assembled and investigated to allow flexible binding and elution conditions. The rationale for the