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The dependence on intracellular ATP concentration of ATP-sensitive K-channels and of Na,K-ATPase in intact HIT-T15β-cells

✍ Scribed by Ichiro Niki; Frances M. Ashcroft; Stephen J.H. Ashcroft

Book ID
115922633
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
469 KB
Volume
257
Category
Article
ISSN
0014-5793

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ATP-sensitive K-channels in HIT T15β-cel
ATP-sensitive K-channels in HIT T15β-cells studied by patch-clamp methods,86Rb efflux and glibenclamide binding
✍ I. Niki; R. P. Kelly; S. J. H. Ashcroft; Frances M. Ashcroft 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 1020 KB

ATP-sensitive K-channels in the cloned beta-cell line HIT T15 were studied by patch-clamp methods; by measurement of 86Rb efflux; and by [3H]glibenclamide binding to isolated membrane preparations. In inside-out patches a 50 pS K-channel was found which was blocked by ATP or tolbutamide applied to t