The DDX1 gene maps within 400 kbp 5′ to MYCN and is frequently coamplified in human neuroblastoma
✍ Scribed by Lukas C. Amler; Jörg Schürmann; Manfred Schwab
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 344 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Human neuroblastoma cells frequently show amplification of the oncogene MYCN, which maps to 2p24. Previous studies have localized the DEAD box motif gene DDXl to the same chromosome band and demonstrated coamplification of DDXl and MYCN in two retinoblastoma cell lines. Recently, a high frequency of coamplification of DDXl and MYCN has been shown in human neuroblastoma cells. We have determined the physical distance between the two genes by pulsed field gel elearophoresis in normal tissue and have found that DDXl maps to a position at a maximum distance of 400 kbp 5' to MYCN. Two neuroblastoma cell lines with coamplification of DDXIIMYCN showed a similar topographic relationship of the two genes. In contrast, in two cell lines with high copy number, the DDXl gene was not present in all amplified units recognized by MYCN and had changed its position in the amplified D N A relative to MYCN from 5' to 3'. presumably by rearrangement during the amplification process. Our data show that the high frequency of DDXl coamplification is due to its close physical distance to MYCN. Althounh amplification has resulted in an elevated expression of DDX I the significance of overexpression for neuro--.