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The cyclic AMP-dependent initiation of DNA synthesis by T51B rat liver epithelioid cells

✍ Scribed by A. L. Boynton; J. F. Whitfield

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
776 KB
Volume
101
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The brief rise in the cellular cyclic AMP content which occurs late in the prereplicative phases of rat hepatocytes in vivo and T51B rat liver epitheloid cells in vitro seems to be necessary for the initiation of DNA synthesis. Thus, the extracellular calcium‐deprivation in T51B rat liver cells in culture which induces a late G‐1 block is rapidly reversible (cells surge into S phase within one hour) either by creating a cyclic AMP surge by the addition of calcium or 3‐isobutyl‐1‐methyl xanthine (a cyclic 3′, 5′‐nucleotide phosphodiesterase inhibitor) or by the exogenous addition of low concentrations of cyclic AMP itself (i.e., 10^−8^‐10^−5^ M). On the other hand, prevention of the calcium‐induced cyclic AMP surge by imidazole (a cyclic 3′, 5′‐nucleotide phosphodiesterase activator) blocked the initiation of DNA synthesis by the calcium‐deprived T51B cells.

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