✦ LIBER ✦

The culturing of dissociated newt forelimb regenerate cells

✍ Scribed by Jabaily, Joseph A. ;Blue, Patricia ;Singer, Marcus

Publisher: John Wiley and Sons
Year: 1982
Tongue: English
Weight: 760 KB
Volume: 219
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402190108

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✦ Synopsis

Abstract

A procedure is described for the mechanical dissociation and culturing of early stage newt regenerate blastema cells. The specified growth medium is adjusted to suitable osmolarity and consists of Leibovitz L‐15, water, fetal calf serum, and insulin. This provides a suitable environment in which growth, proliferation, and some differentiation occur with time as evidenced by phase contrast photographs of living cultures.

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