The culture of human skin fibroblasts on microcarriers in the presence of serum substitute: Ultroser G
โ Scribed by MIECH, G; MYARA, I; MANGEOT, M; GAUTIER, M; LEMONNIER, A
- Book ID
- 122142916
- Publisher
- Portland Press
- Year
- 1990
- Tongue
- English
- Weight
- 256 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0248-4900
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๐ SIMILAR VOLUMES
## New lersey 07 I03 Previously, we demonstrated that removal of fetal bovine serum (FBS) from the medium of human skin fibroblasts resulted in an accelerated '%b' washout, decreased cellular K i , and increased Na' contents. In the present study we examined the mechanism underlying these changes.
Stimulation of an amiloride-sensitive Na+ influx pathway, which mediates Na+/H+ exchange, has been postulated to be an important step in the initiation of DNA synthesis in quiescent human fibroblasts. If the elevation of intracellular Na+ or the alkalinization of intracellular pH resulting from the