β Scribed by Paul, A.G. ;Kelleher, W.J. ;Schwarting, A.E
No coin nor oath required. For personal study only.
Various com ounds representing structural moieties of lysergic acid were added to submergetculrures and to replacement cultures of Ciuviceps, Qualitative studies for probable alkaloid synthesis and quantitative utilization of some of these compounds are reported. The effect on growth and a new method for the detection of alkaloids on paper chromatographs are demonstrated.
A in saprophytic culture date back to Bonns
(1) who succeeded in growing Clawiceps purpurea on a variety of complex media. Extracts of the fungus growth failed to show any physiological activity. Since then various workers (2-8) have reported success in producing the alkaloids. However, either because of cumbersome techniques or insignificant yields, their methods have not been employed commercially.
An understanding of the mechanisms by which the ergot alkaloids are produced, and consequently success in the production of them, might well be demonstrated by attempting t o establish their pathway of synthesis.
The structure common to all of the physiologically active alkaloids is &lysergic acid and in this structure can be seen the semblance of anthranilic acid, indole, tryptophan, amino-Nmethyltryptophan, nicotinic acid, quinolinic acid, pyridoxine, and trigonelline. The compounds represented by these moieties, with the
π SIMILAR VOLUMES
Ultrastructural characteristics of optimally developed seed cultures of two morphologically and biochemically different Claviceps purpurea strains were established. The vegetative mycelium of both conidial (L-17) and mycelial (L-18) strains was filamentous and consisted of cells with a polynuclear s