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The corynebacterial insertion sequence IS31831promotes the formation of an excised transposon fragment

✍ Scribed by Alain A. Vertès; Yoko Asai; Masayuki Inui; Miki Kobayashi; Hideaki Yukawa

Book ID: 104633031
Publisher: Springer Netherlands
Year: 1995
Tongue: English
Weight: 385 KB
Volume: 17
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00128375

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✦ Synopsis

nashiki, /baraki 300-03, Japan Sumptuary The corynebacterial insertion sequence IS31831 was demonstrated to promote in vivo formation of an excised transposon fragment (ETF). The results presented in this report support the view that ETF formation is a general feature of conservative transposition.

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Integration specificity of an artificial kanamycin transposon constructed by the in vitro insertion of an internal Tn5 fragment into IS2

✍ Saint-Girons, I. ;Fritz, H. -J. ;Shaw, C. ;Tillmann, E. ;Starlinger, P. 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 581 KB

IS2 has been marked genetically by the in vitro insertion into its HindIII site of a 3.3 Kb HindIII fragment of Tn5 conferring resistance to kanamycin. The transposition of the IS2::Km, thus obtained, to lambda has been found and insertion sites were characterised. Each of ten independent IS2::Km in