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The conversion of ATP to IMP by muscle surface enzymes

✍ Scribed by C. R. Dunkley; J. F. Manery; E. E. Dryden

Publisher: John Wiley and Sons
Year: 1966
Tongue: English
Weight: 576 KB
Volume: 68
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040680305

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✦ Synopsis

These experiments showed that a n isolated muscle bundle could he used to study, simultaneously, ion transport and the activity of surface enzymes. Frog muscles were carefully dissected and incubated for four hours in Ringer's solution containing a tris buffer and 3 mM ATP; at various times samples of the medium were chromatographed and analysed spectrophotometrically for nucleotide content. Samples of the final medium were analysed for inorganic phoshpate and for ammonia. The results demonstrated the conversion of adenosine triphosphate to inosine monophosphate, via adenosine diphosphate and adenosine monophosphate. Under the conditions of the experiment IMP was detected on chromatograms within 20 minutes of incubation; at the end of four hours the media had concentrations of 2.3 mM IMP, 4.4 mM inorganic phosphate and 2.6 mM ammonia, showing a stoichicmetric rela-

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