The conversion of a RFLP assay into PCR for the determination of purity in a hybrid pepper cultivar

✍ Scribed by Orna Livneh; Eyal Vardi; Yehuda Stram; Orit Edelbaum; Ilan Sela

• Book ID: 104617516
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 377 KB
• Volume: 62
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00037934

✦ Synopsis

A RFLP assay which had been developed for distinguishing between a hybrid pepper and its parents, has been converted into a simple applicable PCR assay, suitable for large-scale quality-control assessment of the commercial hybrid . For this conversion the sequences of the ends of the probe used in the previous RFLP assay were determined . From these sequences suitable primers were devised for inverse PCR of heterogeneous DNA fragments derived from the male parent . The inverse-PCR product was cloned and partially sequenced . These sequences, in turn, made it possible to determine primers on both sides of the locus of mutation, and to develop the reported conventional PCR assay .