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The construction and preliminary analysis of a Tn5 transposon based random mutant library of baculovirus

✍ Scribed by Li, Hui ;Zhao, Minglei ;Yin, Juan ;Zhong, Jiang


Book ID
107382501
Publisher
Higher Education Press and Springer
Year
2006
Tongue
English
Weight
434 KB
Volume
1
Category
Article
ISSN
1673-3509

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A sequence homologous to the conventional nifH gene has been cloned from a different region of the Azotobacter vinelandii genome. Tn5 insertions were obtained in this clone and the mutagenized plasmid was used for marker exchange with A. vinelandii strain CA12 (delta nifHDK) to obtain Tn5 mutants. T