The Conformation of Polycytidylic Acid, Polyguanylic Acid, Polyinosinic Acid, and Their Helical Complexes in Aqueous Solution from Laser Raman Scattering
✍ Scribed by Kenneth G. Brown; Ernest J. Kiser; Warner L. Peticolas
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1973
- Tongue
- English
- Weight
- 63 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
An error in Table I11 listed the ratio of the intensity of the Raman band at 811 cm-l to that at 1098 cm-1 in the poly I-poly C complex as 1.5. This value should have been 1.6. The average maximum value of this ratio for the three helices which can be reversibly ordered in the A form (poly I-poly C, poly A-poly U, and DNA) may then be taken as 1.65 f .5.
A reference to the calculations of Miyazawal on the vibrational frequencies of cis amides-was omitted. His value of 1443 cm-1 for the in-plane N-H (amide 11) vibration in the cis amide diketopiperazine is in reasonable agreement with our assignment of the 1464 cm-1 band in the hypoxanthine base to the in-plane N-H stretch of the cis amide group. In addition his value of 1232 cm-1 for the N-D amide 11' stretch also corresponds to our observation that the 1464 cm-1 band disappears upon deuteration. Thus the assignment of the amide I1 band in hypoxanthine seems well established. However our assignment of the 1330 cm-1 band as the amide 11' band of hypoxanthine must be regarded as conjectural.