The concentration profile of substances separated on thin-media chromatograms

✍ Scribed by Victor Pollak

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 697 KB
• Volume: 77
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)92191-5

✦ Synopsis

Printctl in Tlic Notllcrlancls c HROM. G530 THE CONClSNTRAT1ON PROL'I1JZ OF SUBSTANCE5 SEPARATISD ON THIN-MEDIA CI-IROMATOGRRMS

The present pnpcr brings a theoretical study to determine the concentration profile in a zone of separated substance in thin-layer chromatography. If the solution is applied to the origin as a narrow bancl uniform over the entire width of the substrate, the tlleoretical concentration profile of the zone is a Gaussian curve. If the width of the applied hnd cannot bc neglected, the zone profile becomes the integral of a Gaussian curve. In the cast of the Gaussian profile the amount of solute retained in the zone is proportional to the peak concentration value. Several reasons why concentration protiles obscrvecl in practice may deviate from the Gaussian distribution are discussccl.