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The Concentration and Purification of Diphtheria Toxoid by Polyphosphate Precipitation

✍ Scribed by Jacobs, Morris B. ;Gillis, Roberta Jacobs


Publisher
Elsevier
Year
1951
Weight
295 KB
Volume
40
Category
Article
ISSN
0095-9553

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A simple procedure for the purification of an IgG-type monoclonal antibody by affinity precipitation using Eudragit S-100 is presented. The ligand, a microbial lipase previously used as antigen, was coupled to the polymer at a concentration of 40 mg lipase/g Eudragit. This macroligand was reversibly