The Complexing of Concanavalin A and Glucans

✍ Scribed by Stig R. Erlander

• Book ID: 104599678
• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: English
• Weight: 351 KB
• Volume: 22
• Category: Article
• ISSN: 0038-9056
• DOI: 10.1002/star.19700220909

✦ Synopsis

The complex between concanavalin A and glucans was again analyzed with respect to the recent results reported by Doyle. A discussion of the complexing behavior o f various glucans again illustrates that C-6 and C-4 hydroxyls must be involved in the cornplexing mechanism. Carboxylate groups are most likely not involved in the complex, because 4.2 M NaCl can not prevent the complex from forming. That is, i f carboxylate ions were involved, such concentrated Na+ ions would act as inhibitors to the reaction with more strength than neutral molecules such as D-mannose. Carboxylate groups are most likely chelated to Mn++ ions and hence are involved in the stabilization of the protein tetramer. Such complexing as well as the folding of the protein could account for changes in pK and inactivation of these groups.