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The cleavage site of restriction endonuclease MnlI

✍ Scribed by Brinkley, Pamela; Bautista, Diosdado S.; Graham, Frank L.


Book ID
123386941
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
221 KB
Volume
100
Category
Article
ISSN
0378-1119

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MnlIβ€”The member of H-N-H subtype of Type
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The Type IIS restriction endonuclease MnlI recognizes the non-palindromic nucleotide sequence 5V-CCTC(N)7/6, and cleaves DNA strands as indicated by the arrow. The genes encoding MnlI restriction -modification system were cloned and sequenced. It comprises N6methyladenine and C5-methylcytosine methy