The Cis-specificity of the Q-gene product of bacteriophage lambda

✍ Scribed by Burt, D. W. ;Brammar, W. J.

• Book ID: 104727185
• Publisher: Springer
• Year: 1982
• Tongue: English
• Weight: 485 KB
• Volume: 185
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00334142

✦ Synopsis

A trp/lacW205 substitution, fused to the late region of bacteriophage lambda, provided a convenient assay for phage late gene expression in the presence or absence of lambda pQ. Comparison of lacZ expression from Q+ and Q- phages showed that late gene expression was markedly Q-dependent (263-fold difference). A cis/trans comparison of lambda pQ action showed a 180-fold difference in lacZ expression. The results suggest that pQ in only significantly active when supplied in cis to its site of action.