Continuous spectrophotometric assay for
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Timothy R. Waters; Bernard A. Connolly
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Article
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1992
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Elsevier Science
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English
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A continuous spectrophotometric assay for the EcoRV restriction endonuclease has been developed. The synthetic self-complementary oligonucleotide d(GACGATATCGTC) (which is double stranded under the assay conditions) is used as the substrate. The EcoRV endonuclease recognizes d(GATATC) sequences cutt