✦ LIBER ✦
The chromatography of nucleic acid preparations on deae-cellulose paper : III. Study of depolymerization and denaturation processes
✍ Scribed by P. Charles; L. Ledoux
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 666 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
The conditions of the CPP fractionation were the same in all the cases. DNA was incubated with the DNAase in the presence of 5.10-a M MgCl, in a 0.01 M phosphate buffer ( pH 7.8) at 37O, At o, 20, 40 and60 PAPER CHROMATOGRAPHY OF NUCLEIC ACID PREPARATIONS. III. ' molecules. During the melting phenomenon by heat denaturation, the different fractions of calf thymus DNA are modified in such a way that they are eluted with eluants 5 and 7. At a given temperature (when equal or superior to the melting point), the relative J. Ckromalog., 25 (1966) 135-143 PAPER CHROMATOGRAPHY OF NUCLEIC ACID PREPARATIONS. III.