The chemotactic activity for granulocytes produced by virally infected fibroblasts is identical to monocyte-derived interleukin 8
✍ Scribed by Jo Van Damme; Benny Decock; Renè Conings; Jean-Pierre Lenaerts; Ghislain Opdenakker; Alfons Billiau
- Book ID
- 102826651
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 762 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
The chemotactic activity for granulocytes produced by virally infected fibroblasts is identical to monocytederived interleukin 8* So far, the role of fibroblasts in inflammatory processes has been underestimated. We have previously shown that stimulation of fibroblasts with viruses or bacteria results in a simultaneous production of several cytokines, including interferon$, interleukin (IL) 6 and colony-stimulating factors. We here report that virally infected fibroblasts produce also a chemotactic factor for granulocytes. The activity is inducible not only by measles virus but also by IL l p and the double-stranded RNA poly(r1). poly(rC). This factor, when purified to homogeneity, occurs as a 6-7-kDa protein doublet upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The pure protein is serologically related to a fully characterized granulocyte chemotactic peptide (GCP) from monocytes, designated IL 8. Furthermore, the chemotactic factor from fibroblasts has an NH2-terminal sequence identical to that of GCP/IL 8, small differences in NH2-terminal processing being observed. Finally, in addition to diploid fibroblasts, the osteosarcoma MG-63 cell line is also a producer of GCP/IL8. It can thus be concluded that GCP/IL8 can be produced by several cell types in response to infection and that fibroblasts can contribute to chemotaxis in inflammation.