The chemokine SDF-1α triggers a chemotactic response and induces cell polarization in human B lymphocytes
✍ Scribed by Miguel Vicente-Manzanares; María C. Montoya; Mario Mellado; José M. R. Frade; Miguel A. del Pozo; Marta Nieto; Manuel O. de Landazuri; Carlos Martínez-A.; Francisco Sánchez-Madrid
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 214 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
We studied the expression and possible functional role of chemokine receptors CXCR3, CXCR4 and CCR5 in normal human B lymphocytes. B cells from both peripheral blood and tonsils expressed high levels of CXCR4 but not the other chemokine receptors tested. CXCR4 ligand, stromal cell-derived factor (SDF)-1 § , elicited a potent chemotactic response and induced a polarized motile phenotype in B cells, resulting in redistribution of the adhesion molecule ICAM-3 to a posterior appendage of the cell, termed uropod, and of CXCR4 receptor to the leading edge of migrating B cells. Time-lapse videomicroscopy studies revealed that SDF-1 § -treated cells recruited additional bystander B cells through the uropod. SDF-1 § induced levels of cellular recruitment comparable to those elicited by polarization-inducing anti-ICAM-3 monoclonal antibody, in an LFA-1/ICAM-1, -3-dependent fashion. Moreover, this chemokine increased intracellular Ca 2+ levels in B lymphocytes, and induced a rapid CXCR4 receptor down-regulation on the cell surface membrane. These results provide new insight into the important biological role of SDF-1 § in physiological processes in which B cells participate, and suggest a key role for chemokines in normal B cell trafficking and recirculation.