The acyl-enzyme formed upon acylation of a-chymotrypsin with isatoic anhydride has been characterised by infrared spectroscopy. Acylation at pH 7 to yield the 2-aminobenzoyl-enzyme is rapid (k 5.57 Â 10 À2 s À1 ), while deacylation is much slower (k 3.7 Â 10 À5 s À1 ). The [ 13 CO]-labelled form of
The charge relay system in chymotrypsin-IR studies of models for the hydrogen Bonds
✍ Scribed by Georg Zundel
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 977 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0022-2860
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