The characterization of non-histone proteins whose amounts increase in chromatin from mouse hepatocarcinomas
✍ Scribed by Zh. A. Medvedev; J. H. Buchanan; M. N. Medvedeva; H. M. Crowne
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- French
- Weight
- 600 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
A fraction containing liver‐ and hepatoma‐specific nonhistone proteins has been isolated from the chromatin of mice. Amino acid analysis of this fraction shows that it contains 16 mol of glutamic acid, 10 mol aspartic acid, 7 mol of both arginine and lysine per 100 mol and contains no cysteine or tyrosine. The proteins in this fraction are strongly associated with DNA and are co‐extracted with histones from chromatin with 0.25 M HCI. In chromatin from age‐related hepatomas, the amount of this fraction increased six‐fold. This increase in concentration of these chromatin proteins may be associated with changes of chromatin structure necessary to initiate malignant growth in liver cells.