The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100
✍ Scribed by Mirosława Panasiewicz; Hanna Domek; Grażyna Hoser; Natalia Fedoryszak; Maciej Kawalec; Tadeusz Pacuszka
- Book ID
- 111490449
- Publisher
- SP Versita
- Year
- 2009
- Tongue
- English
- Weight
- 499 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1425-8153
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✦ Synopsis
Abstract
Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)