The centromere kinesin-like protein, CENP-E. An autoantigen in systemic sclerosis

✍ Scribed by Jerome B. Rattner; Jennifer Rees; Frank C. Arnett; John D. Reveille; Rose Goldstein; Marvin J. Fritzler

• Book ID: 102754426
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 678 KB
• Volume: 39
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780390813

✦ Synopsis

Autoantibodies directed against centromere proteins (CENPs) are a serologic feature in some patients with systemic sclerosis (SSc). Previous studies have focused on autoantibodies to CENPs A, B, and C. CENP-E is a recently described 312-kd protein that also localizes to the centromere. Therefore, we studied the presence of autoantibodies to recombinant CENP-E in patients with SSc.

Methods. Sixty sera from patients with the SSc spectrum of diseases were screened for the presence of autoantibodies against CENP-E, by indirect immunofluorescence and immunoblotting using recombinant CENP-E protein. HLA class I1 alleles were determined by DNA oligotyping.

Results. Among the SSc sera, 15 of 60 (25%) demonstrated antibody reactivity with recombinant CENP-E, and 14 of these 15 sera (93%) had antibodies directed against another CENP. Anti-CENP-E was seen in 13 of 30 sera with anti-CENP (43%). All patients with anti-CENP-E had a limited form of SSc, known as the CREST variant (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias).