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The cellobiohydrolase I from Trichoderma reesei QM 9414: action on cello-oligosaccharides

✍ Scribed by Mária Vrs̆anská; Peter Biely

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 673 KB
Volume: 227
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(92)85058-8

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✦ Synopsis

The action of cellobiohydrolase I [(1+4)-/I-n-glucan cellobiohydrolase, EC 3.2.1.91, CBH-I] from Trichoderma reesei QM 9414 on cello-oligosaccharides labelled with tritium in the reducing moiety has been investigated. There was a marked preference of CBH-I to attack the first three linkages at the reducing ends of the cello-oligosaccharides (cellotriose+ellohexaose). [ I-'H]Cellobiose and Cmethylumbelliferyl /?-Dglucopyranoside served as glycosyl acceptors during the degradation of cellotriose by CBH-I.

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✍ Javier Jiménez; Juan Manuel Domínguez; María Pilar Castillón; Carmen Acebal 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 651 KB

Irreversible thermoinactivation of cellobiohydrolase I from Trichoderma reesei has been analyzed at 70°C and pH 4.8. The time course of thermal inactivation and the dependence of the inactivation rates on protein concentration suggested that aggregation followed by precipitation was the main process