The CD of two-chain coiled coils: Experiments on tropomyosin and tropomyosin segments in the tyrosin/disulfide spectral region

✍ Scribed by Marilyn Emerson Holtzer; Swatantar Kumar; Alfred Holtzer; Dan L. Crimmins

• Publisher: Wiley (John Wiley & Sons)
• Year: 1989
• Tongue: English
• Weight: 939 KB
• Volume: 28
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360280909

✦ Synopsis

Synopsis

CD experiments are reported for several coiled-coil species in the tyrosine/disulfide (-250-350-nm) region. Intact noncross-linked tropomyosin ( -3°C) shows a negative nonsymmetric band maximal at 280 nm. This spectrum is the sum over six tyrosines/chain, and has conformational significance, since it disappears on denaturation. Experiments on an excised coiled-coil segment, each of whose chains comprise residues 11-127 of the tropomyosin sequence and only one tyrosine (Y60), reveal that not all tyrosines are alike. The spectrum at 3°C shows a small negative maximum at -285 nm and a substantial, hitherto unknown, positive band at -270 nm, the latter masked in the parent protein by the negative contribution from the other tyrosines. A noncross-linked coiled-coil segment comprising residues 142-281, in which Y60 is absent, shows no such positive band. This peculiarity of Y60 is confirmed by absorbance spectra, with the extinction coefficient of Y60 larger in benip media than the average of the other tyrosines. Intact (3°C) C190 cross-linked tropomyosin is known to yield, besides tyrosine contributions, a positive maximum at -300 nm. Subtracting the corresponding data for noncross-linked tropomyosin shows that the disulfide spectrum itself actually has two equal, partly resolved bgnds at, respectively, 250 and 280 nm. The existence of a chiral disulfide argues for a relatively rigid, perhaps strained, local coiled coil. A C190 cross-linked segment comprising residues 142-281 shows a chiral disulfide spectrum like tropomyosin's, but another segment, comprising residues 168-284, shows none; thus removal of residues 142-167 causes loss of chirality at C190, over 20 residues away. These spectra thus contain important information on the subtle local differences in coiled-coil structures.