The canonical T cell receptor of dendritic epidermal γδ T cells is highly conserved between rats and mice
✍ Scribed by Petra Kühnlein; Rita Mitnacht; Nora E. Torres-Nagel; Thomas Herrmann; Adelheid Elbe; Thomas Hünig
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 548 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
The canonical T cell receptor of dendritic epidermal y 6 T cells is highly conserved between rats and mice Two monoclonal antibodies with specificity for rat y6 T cell receptor (TCR) were generated. One, called V65, reacts with all CD3' a@ TCR-rat Tcells and thus recognizes a constant determinant of the rat y 6 TCR (Kuhnlein et al., Journal of Immunology 1994, 153 : 979). The other, called V45, reacts with approximately 80 YO of y 6 T cells in peripheral lymphoid organs. In rat epidermis, V65 but not V45 detects a dense network of the dendritic epidermal Tcells (DETC). Analysis of epidermal RNA by polymerase chain reaction (PCR) indicated that Vy3 and V61 are the predominant, if not exclusive TCR V transcripts present at this site. Sequence analysis of cDNA clones obtained by reverse transcription-PCR with Vy3-and V61-specific primers revealed that the variable domains of rat DETC y and 6 chains are very homologous to those described in mice (92 YO and 95 % identity at the protein level). The complete conservation between the two species of the amino acid sequences at the V-(D)-J transitions of this monomorphic receptor indicates that the interaction of the DETC TCR with its as yet unknown ligand must be of central importance for DETC function.