The butadiene metabolite, 1,2:3,4-diepoxybutane, induces micronuclei but is only weakly mutagenic at lacI in the Big Blue® Rat2 lacI transgenic cell line
✍ Scribed by Christopher J. Saranko; Leslie Recio
- Book ID
- 101265040
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 118 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
1,3-Butadiene (BD) is a genotoxic carcinogen that media or solvent controls. However, regression is bioactivated to at least two mutagenic metabo-analyses indicated a trend toward increasing mutant lites, 1,2-epoxybutene (EB) and 1,2:3,4-diepoxybu-frequency with increasing DEB exposure concentratane (DEB). We investigated the mutagenicity and tion. Experiments to examine the induction of miinduction of micronuclei by DEB in vitro in Rat2 cronuclei by DEB revealed a concentration-dependent lambda/lacI transgenic fibroblasts (Big Blue Rat2 increase in micronuclei in Rat2 cells following expocells, Stratagene, LaJolla, CA). Assays for mutage-sure to DEB. These results indicate that DEB induces nicity and micronuclei induction were carried out micronuclei in the absence of detectable gene mutaat concentrations of 0, 2, 5, or 10 mM DEB for 24 tion at lacI in Big Blue Rat2 cells. The induction of hours. Exposure of cells to these concentrations of micronuclei but only weak mutagenicity at the lacI DEB resulted in approximately 100, 50, and 10% transgene is likely due to the poor recovery of delesurvival, respectively, compared with media con-tions using this lambda shuttle vector system, demontrols. In independent replicate experiments, no sta-strating the need to investigate multiple endpoints of tistically significant increase in lacI mutant fre-genotoxicity when considering the mutational activquency was observed in Rat2 cells at any of the ity of a compound. Environ. Mol. Mutagen.