The bradykinin BK2 receptor mediates angiotensin II receptor type 2 stimulated rat duodenal mucosal alkaline secretion

✍ Scribed by Sara Ewert; Berndt Johansson; Mathias Holm; Herbert F Helander; Lars Fandriks

• Book ID: 104497870
• Publisher: BioMed Central
• Year: 2003
• Tongue: English
• Weight: 700 KB
• Volume: 3
• Category: Article
• ISSN: 1472-6793
• DOI: 10.1186/1472-6793-3-1

✦ Synopsis

Background:

This study investigates bradykinin and nitric oxide as potential mediators of at2-receptor-stimulated duodenal mucosal alkaline secretion. duodenal mucosal alkaline secretion was measured in methohexital- and alpha-chloralose-anaesthetised rats by means of in situ ph-stat titration. immunohistochemistry and western blot were used to identify the bk2 receptors.

Results:

The at2 receptor agonist cgp42112a (0.1 microg kg(-1) min(-1)) administered intravenously increased the duodenal mucosal alkaline secretion by approximately 50 %. this increase was sensitive to the selective bk2 receptor blocker hoe140 (100 ng/kg i.v.), but not to luminal administration of the nos blocker l-name (0.3 mm). mean arterial pressure did not differ between groups during the procedures. immunohistochemistry showed a distinct staining of the crypt epithelium and a moderate staining of basal cytoplasm in villus enterocytes.

Conclusion:

The results suggest that the at2-receptor-stimulated alkaline secretion is mediated via bk2 receptors located in the duodenal cryptal mucosal epithelium.