The bovine major histocompatibility complex(BoLA):Close linkage of the genes controlling serologically defined antigens and mixed lymphocyte reactivity
✍ Scribed by William R. Usinger; Martin Curie-Cohen; Kathy Benforado; Deborah Pringnitz; Robert Rowe; Gary A. Splitter; William H. Stone
- Publisher
- Springer-Verlag
- Year
- 1981
- Tongue
- English
- Weight
- 344 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0093-7711
No coin nor oath required. For personal study only.
✦ Synopsis
Detection of linkage between genetic loci in cattle has been hampered by the lack of large full-sib families. A unique source of full-sib families is now available from embryo transplantation. Lymphocytes from six full-sib families, ranging in size from three to seven siblings, were tested for serologically defined BoLA antigens (BoLA-A). In addition, mixed lymphocyte reactivity (MLR) was tested between all paired combinations of cells within each family to distinguish BoLA-D specificities. Serologically identical siblings within each family were reciprocally nonreactive in MLR, and vice versa; thus, no recombinants were detected between the BoLA-A and the BoLA-D loci. Classical genetic linkage analysis revealed that these loci are significantly closer than 11.9 centimorgans.