The biosynthesis of cytokinins in crown-gall tissue ofVinca rosea
โ Scribed by T. Stuchbury; L. M. Palni; R. Horgan; P. F. Wareing
- Publisher
- Springer-Verlag
- Year
- 1979
- Tongue
- English
- Weight
- 554 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
โฆ Synopsis
The crown-gall tissue of Vinca rosea converts labelled adenine into cytokinins. The principal initial products appear to be ribosylzeatin phosphates; zeatin and ribosylzeatin are also produced in appreciable quantities. The efficiency of conversion of adenine into cytokinins suggests a pathway of synthesis independent of turnover of tRNA. Isopentenyl adenine or its derivatives do not appear to be intermediates in the conversion of adenine to zeatin compounds. Cytokinins in V. rosea turnover rapidly and further metabolism of zeatin derivatives seems to result in their conversion into glucosides which are the main cytokinin active compounds in the tissue.
๐ SIMILAR VOLUMES
An improved method for the detection of octopine in Crown-gall tissues and in shoots regenerated from these tissues has been devised using [14C]arginine as a precursor. By using bidimensional chromatography followed by autoradiography, very small amounts of octopine can be clearly detected in tumors