The biosynthesis of chlorophyll in greening barley (Hordeum vulgare). Is there a light-independent protochlorophyllide reductase?
✍ Scribed by Klaus Apel; Michael Motzkus; Katayoon Dehesh
- Publisher
- Springer-Verlag
- Year
- 1984
- Tongue
- English
- Weight
- 496 KB
- Volume
- 161
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Recently, some evidence for the occurrence of a light-independent protochlorophyllidereducing enzyme in greening barley plants has been presented. In the present work this problem was reinvestigated. 6-[14C] Aminolevulinic acid was fed to isolated barley shoots from plants which had been preilluminated for various lengths of time. Porphyrins which had been synthesized during the dark incubation were analyzed by high-performance liquid chromatography. There was no evidence for a light-independent synthesis of chlorphyll(ide). The 14C-labelled precursor was incorporated almost exclusively into protochlorophyllide. The reduction of labelled protochlorophyllide to chlorophyllide was strictly light-dependent. These results are not consistent with the existence of a light-independent protochlorophyllide-reductase in barley as proposed previously.