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The behavior in vitro of tissues from lethal embryos

โœ Scribed by Ephrussi, Boris

Publisher
John Wiley and Sons
Year
1935
Tongue
English
Weight
407 KB
Volume
70
Category
Article
ISSN
0022-104X

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โœฆ Synopsis

The problem of tlie effect of letlial gciies 011 development is oiic of considerable interest from tlie geiictical as ~vcll as from the embryological poiiit s of view. The โ‚ฌew investigations alreatly pnblislied seem to jriclicatc that letlial genes may affect development in very different ways,

The application of tissue cwltnre to tlie study of lethal embryos, attemptcd ill this and in a prwions study (Eplirwsi, ' 3 3 ) , is based on the assumption that, this technic s4ioiild permit the dctermina~ioii of the poteiitialities of the cells of ail embryo which is non-viable in vivo. It should be possible by means of tliis method to solve several preliminary ques-

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Having found that tissues from chick embryos grow readily in artificial media, nutrient agar and bouillon,l the next step was the attempt to cultivate such tissues in media all the constituents of which are known. In collaboration with Dr. Arthur Koelker we were able to obtain excellent growth in co