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The autoradiographic test for unscheduled DNA synthesis: a sensitive assay for the detection of DNA repair in the HepG2 cell line

✍ Scribed by Isabelle Valentin-Severin; Véronique Thybaud; Anne-Marie Le Bon; Jean-Claude Lhuguenot; Marie-Christine Chagnon


Book ID
116758772
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
107 KB
Volume
559
Category
Article
ISSN
1383-5718

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A modified assay for the detection of DNA single-strand breaks (SSBs) in human mononucleated white blood cells (MWBCs) based on the nick translation (NT) reaction was developed and combined with the test for unscheduled DNA synthesis (UDS). Both assays were performed on disposable 96-well filtration