Acetyl-CoA synthetase activity in vitro is assayed quickly and conveniently by incubating whole chloroplasts, chloroplast extracts, or leaf extracts with labeled acetate, CoA, ATP, and Mg and transferring aliquots of the reaction mixture to pieces of either Whatman No. 1 or DE81 filter paper. Unreac
The association of gibberellin-like activity with the chloroplast fraction of leaf homogenates
β Scribed by J. L. Stoddart
- Publisher
- Springer-Verlag
- Year
- 1968
- Tongue
- English
- Weight
- 386 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
Significant gibberellin-like activity has been detected in the 1000 X g fraction of leaves of Brassica oleracea var. acephala (Canson kale) and Hordeum vulgate (cv. "Himalaya"). In kale and barley the qualitative pattern of activity found in the chloroplast fraction differs from that normally seen in total-leaf extracts. When expressed on a total chlorophyll or sample fresh-weight basis, approximately 16 % of the gibberellin-like activity found in the leaf can be accounted for in the 1000 β’ g or chloroplast fraction. The physiological implications of this finding are discussed.
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