The Assay of Digoxin Preparations*

✍ Scribed by Banes, Daniel

• Publisher: Elsevier
• Year: 1954
• Weight: 300 KB
• Volume: 43
• Category: Article
• ISSN: 0095-9553
• DOI: 10.1002/jps.3030430610

✦ Synopsis

A method is proposed for the determination of digoxin, based upon the color developed with m-dinitrobenzene in weakly alkaline solution. Gitoxin does not interfere when present in concentrations lower than 8 per cent.

HE PRESCRIBED ASSAY method for digoxin "preparations official in u. s. P. XIV (1) depends upon the blue color produced when mdinitrobenzene reacts with the butenolide ring in the presence of strong alkali. The color is developed at 0 to 2', and its absorbancy is measured in the spectrophotometer exactly five minutes after the addition of the alkali. But a t 0"