The assay of ATP-sulfurylase

✍ Scribed by Frits A. Hommes; Linda Moss

• Book ID: 102627810
• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 249 KB
• Volume: 154
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90501-4

✦ Synopsis

An assay method for ATP-sulfurylase is described, based on the incorporation of 35SO4(2-) into adenosine-5'-phosphosulfate (and 3-phosphoadenosine-5'-phosphosulfate) and the separation of these compounds by HPLC. Since the enzyme is easily inactivated at temperatures above 20 degrees C during the assay, the reaction time should not exceed 10 min.