The application of the laser induced proton pulse for studying electroneutral ion exchange across biomembranes
✍ Scribed by Menachem Gutman; Esther Nachliel
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 456 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0013-4686
No coin nor oath required. For personal study only.
✦ Synopsis
An electroneutral ion exchange across a membrane cannot generate an electric signal. Yet on a molecular level the reaction consists of steps where surface charges are formed and neutralised. Thus with proper synchronisation and time resolution the electrosilence should be broken. Using the laser induced proton pulse as synchronizing perturbation, we monitored the capacitance current associated with H+-Na+ exchange by monensin impregnated in a phospholipid membrane.
A global kinetic analysis, based on numerical solution of coupled non linear differential rate equations, reconstructed the observed current and the charge dynamics on each side of the membrane.