The anticlastogenic effect of various combinations of cysteamine, AET, HCT, and amino acids on chromosome damage by Trenimon and bleomycin in human lymphocytes in vitro
✍ Scribed by Erich Gebhart
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 940 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0340-6717
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✦ Synopsis
The protective activity of the combined application of anticlastogens against the chromosome-damaging action of Trenimon and bleomycin was studied by analyzing more than 32000 metaphases from cultures of human peripheral lymphocytes. Screening tests with the combinations cysteine/cysteamine/AET, cysteine/methionine/asparagine, cysteine/serine/HCT, and AET/HCT, with Trenimon as clastogen, in no case revealed a hyperadditive (synergistic) effect. From the results of detailed analyses of the action of the combination AET/HCT it was concluded that the (nonsynergistic) anticlastogenic effects observed were induced due to intracellular biologic mechanism, and not due to a reaction in the culture fluid between clastogens and anticlastogens. Although the observations gained with Trenimon or bleomycin differed in some respects, the anticlastogens apparently act via a mechanism at least common to AET and HCT, i.e., they manifest their effect in lymphocyte cultures by a limited interaction with the process of aberration formation, rather than by influencing repair processes, which are blocked by caffeine.