The anti-invasive effect of lucidenic acids isolated from a new Ganoderma lucidum strain

✍ Scribed by Chia-Jui Weng; Chi-Fai Chau; Kuang-Dee Chen; Deng-Hai Chen; Gow-Chin Yen

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 769 KB
• Volume: 51
• Category: Article
• ISSN: 1613-4125
• DOI: 10.1002/mnfr.200700155

✦ Synopsis

Abstract

Ganoderma lucidum is a well‐known mushroom with various pharmacological effects that has been used for health and longevity purposes. The objective of this study was to investigate the anti‐invasive effect of lucidenic acids isolated from a new G. lucidum strain (YK‐02) against human hepatoma carcinoma (HepG~2~) cells. Triterpenoid components in the ethanol extract of G. lucidum (YK‐02) were separated by means of a semi‐preparative RP HPLC. Four major peaks were separated and crystallized from triterpenoids fraction, and were identified as lucidenic acids A, B, C, and N according to their spectroscopic values of ^1^H NMR and MS. Treatment of the lucidenic acids (50 μM) in the presence of 200 nM phorbol 12‐myristate 13‐acetate (PMA) after 24 h of incubation all resulted in significant inhibitory effects on PMA‐induced MMP‐9 activity and invasion of HepG~2~ cells. The results indicate that the lucidenic acids isolated from G. lucidum (YK‐02) are anti‐invasive bioactive components on hepatoma cells.