The analytical pyrolysis of carbohydrates. 2—differentiation of homopolyhexoses according to their linkage type, by pyrolysis–mass spectrometry and pyrolysis-gas chromatography/mass spectrometry

✍ Scribed by Arie van der Kaaden; Jaap J. Boon; Johan Haverkamp

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 640 KB
• Volume: 11
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200110910

✦ Synopsis

Curiepoint pyrolysis(Pykmass spectrometry (14 eV electron impact) in various modes of operation and Curiepoint pyrolysis-gas chromatography/mass spectrometry (Py-CCIMS) afforded discrimination between homopolyhexoses of different glycosidic linkage type and configuration: -a-, (1 -+ 6)-p-glucan and (1 -+ 2)-ar-manaan. F'yrolysis-mass spectrometry in multichannel averaging mode, combined with discriminant analysis data processing, revealed significant discrimination between all polyhexoses, except for the (1 --* 6) linked polymers. By using preheated telescopic glass tube sample holders, additional specificity of the pyrolysis-mass spectral fingerprint for the (1 + 3) linked carbohydrate was obtained. Pyrolysis-mass spectrometry in time-resolved mode yielded ion profiles, especially characteristic for the (1 -+ 3)-@-glucan. Py-GUMS was an additional discriminating dimension (retention time), providing structural information about the pyrolysate constituents, in particular concerning the anhydrohexoses.