The analysis of the binding of two ligands by an allosteric protein
β Scribed by R.F. Steiner; J. Osborne; K. Flanigan
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 811 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0022-5193
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π SIMILAR VOLUMES
The thermodynamic relationship between the binding of the ligand, A-B, and analogs of its groups, A-X and B-Y, to a protein is derived using a thermodynamic cycle. Using the formalism, thermodynamic data, and simple models the following conclusions are reached: (1) A significant free energy barrier
Fluorescence correlation spectroscopy (FCS) is a well-established method for the analysis of freely diffusing fluorescent particles in solution. In a two-colour setup, simultaneous detection of two different dyes allows the acquisition of both the autocorrelation of the signal of each channel and th