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The analysis of low levels of γ-glutamyltransferase activity by high-performance liquid chromatography with electrochemical detection

✍ Scribed by John A. Hoskins; Femi F. Davies

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
367 KB
Volume
152
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for the analysis of low levels of the enzyme y-ghrtamyltransferase in biological samples by high-performance liquid chromatography with electrochemical detection is described. A y-glutamyl moiety from ghrtathione is transferred by the enzyme to glycylglycine to produce a tripeptide which is assayed directly after a purification step using octadecylsihca. Confirmation of the method is by use of the inhibitor AT-125. The method is used to measure the level of enzyme activity in rodent tissues and in cultured cells. 0 1986 Academic PESS, h.

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