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The amylase gene-enzyme system of fishes. I. Developmental expression of amylase in the mosquitofish

✍ Scribed by Yardley, Darrell G.

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
809 KB
Volume
245
Category
Article
ISSN
0022-104X

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✦ Synopsis

The objective of this study is to examine the effects of developmental stage on amylase expression in the mosquitofish (Garnbusia affinis holbrooki, Poeciliidae). Expression was characterized in terms of amylase activity and amount of amylase protein in five developmental stages: early, mid, and late embryos, 5-day-old juveniles, and adults. Two measures of activity were used: a starch-iodine assay, which measures the change in substrate, and the dinitrosalicyclic acid (DNSA) assay, which measures the change in product. To measure changes in the amount of amylase, the enzyme-linked immunosorbent assay (ELISA) was used. Highest activity and amount was observed in the adult stage. In general, both increased with development. While the two activity measures were positively correlated, these were only weakly correlated with the amount of amylase. Possible reasons for these weak correlations between amylase activity and amount are discussed.

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