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The amino-acid residues on the C-terminal side of the cleavage site of angiotensinogen influence the species specificity of reaction with renin

โœ Scribed by James Burton; Timothy Quinn


Book ID
113266867
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
327 KB
Volume
952
Category
Article
ISSN
0167-4838

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We have studied the cleavage efficiency of the protease enterokinase (EK) using the novel vector pESP4. pESP4 is a yeast expression vector equipped with ligation-independent cloning sites, a GST purification tag, and a FLAG epitope tag. EK is used to cleave the FLAG and GST tags leaving the protein